Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Detect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB.

Western blot: 1-10 µg/mL using ECL.

Immunohistochemistry: not tested. It is recommended that the antibody be tried at 1-20 µg/mL on paraformaldehyde fixed tissue.

ELISA: 0.5-1 µg/mL using 1 µg/mL control peptide (Catalog number AG394) per well.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 20 amino acid peptide sequence near the extracellular N-terminus of rat ASIC1 (Chen et al. 1998; Waldmann et al. 1997; Garcia-Anoveros et al. 1997; Waldmann et al. 1996).

The immunogen sequence has 100% homology with human sodium channel 2 or BNaC2.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.

Specificity

Recognizes rat Acid Sensitive Ion Channel 1 (ASIC1). The immunogen shows no significant sequence homology with ASIC-beta or other ASICs.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.